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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
05/06/2018 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CASTRO, M.B.; GRACINDOA, C.V.; LANDIA, M.F.A.; ABRAL FILHOA, S.L.S.; RESENDE FILHOA, N.J.; LIMAA, E.M.M.; RIET-CORREA, F. |
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Afiliación : |
MÁRCIO B. CASTROA, College of Agronomy and Veterinary Medicine, University of Brasília (UnB), Veterinary Teaching Hospital.; CRISTIANE V. GRACINDOA, College of Agronomy and Veterinary Medicine, University of Brasília (UnB), Veterinary Teaching Hospital,Brasil.; MARINA F.A. LANDIA, College of Agronomy and Veterinary Medicine, University of Brasília (UnB), Veterinary Teaching Hospital.; SÉRGIO L.S. CABRAL FILHOA, College of Agronomy and Veterinary Medicine, University of Brasília (UnB), Veterinary Teaching Hospital.; NAPIER J. RESENDE FILHOA, College of Agronomy and Veterinary Medicine, University of Brasília (UnB), Veterinary Teaching Hospital.; EDUARDO M.M. LIMAA, College of Agronomy and Veterinary Medicine, University of Brasília (UnB), Veterinary Teaching Hospital.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Sheep adaptation management, and investigation of inherited resistance to prevent Brachiaria spp. poisoning. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Small Ruminant Research, v. 158, p. 42-47, 2018. |
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DOI : |
10.1016/j.smallrumres.2017.12.001 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 22 August 2017; Received in revised form 30 November 2017; Accepted 1 December 2017;Available online 09 December 2017. |
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Contenido : |
Abstract: Brachiaria spp. is the most used forage in extensive cattle production in the Central-Western region of Brazil. However, livestock fed with these grasses can develop hepatogenous intoxication due to the presence of steroidal saponins that promote photosensitivity, inefficiency of production in the herds and death. Grass toxicity depends
on factors related to animal susceptibility or resistance and intrinsic plant factors, providing significant differences in the clinical manifestation and mortality of livestock. Three experiments were conducted to investigate the adaptation management and inherited resistance to prevent Brachiaria spp. poisoning in sheep. In the first
experiment, the adaptive management of lambs was performed, controlling the grazing time in Brachiaria spp. pastures. Groups of sheep grazing previously in Brachiaria decumbens paddocks for 2 h daily or on alternate days for 60 days presented low hepatotoxic changes and had reduced the number of intoxicated lambs when compared
to the control group during the challenge. In experiment 2, ruminal transfaunation from adapted adult sheep to non-adapted lambs to grazing on the grass reduced the hepatotoxicity, which suggests that Brachiaria spp. poisoning resistance could be transferred by ruminal fluid to non-adapted animals. In the third experiment, lambs from flocks raised in Brachiaria pastures showed less susceptibility to poisoning than lambs from flocks raised in non-toxic pastures, suggesting inherited resistance to the toxicosis. These results suggest that the
control of grazing time combined with transfaunation can be employed to decrease the frequency of poisoning in susceptible flocks. However, a definitive solution in the long-term would be the selection of resistant sheep herds. MenosAbstract: Brachiaria spp. is the most used forage in extensive cattle production in the Central-Western region of Brazil. However, livestock fed with these grasses can develop hepatogenous intoxication due to the presence of steroidal saponins that promote photosensitivity, inefficiency of production in the herds and death. Grass toxicity depends
on factors related to animal susceptibility or resistance and intrinsic plant factors, providing significant differences in the clinical manifestation and mortality of livestock. Three experiments were conducted to investigate the adaptation management and inherited resistance to prevent Brachiaria spp. poisoning in sheep. In the first
experiment, the adaptive management of lambs was performed, controlling the grazing time in Brachiaria spp. pastures. Groups of sheep grazing previously in Brachiaria decumbens paddocks for 2 h daily or on alternate days for 60 days presented low hepatotoxic changes and had reduced the number of intoxicated lambs when compared
to the control group during the challenge. In experiment 2, ruminal transfaunation from adapted adult sheep to non-adapted lambs to grazing on the grass reduced the hepatotoxicity, which suggests that Brachiaria spp. poisoning resistance could be transferred by ruminal fluid to non-adapted animals. In the third experiment, lambs from flocks raised in Brachiaria pastures showed less susceptibility to poisoning than lambs from flocks raised in non-toxic pastures, suggesting inheri... Presentar Todo |
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Palabras claves : |
PLATAFORMA SALUD ANIMAL; RESILIENCE OVINE; SIGNAL GRASS; TOXICITY RESISTANCE. |
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Thesagro : |
OVEJA; OVINOS. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02790naa a2200289 a 4500
001 1058667
005 2019-10-09
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.smallrumres.2017.12.001$2DOI
100 1 $aCASTRO, M.B.
245 $aSheep adaptation management, and investigation of inherited resistance to prevent Brachiaria spp. poisoning.$h[electronic resource]
260 $c2018
500 $aArticle history: Received 22 August 2017; Received in revised form 30 November 2017; Accepted 1 December 2017;Available online 09 December 2017.
520 $aAbstract: Brachiaria spp. is the most used forage in extensive cattle production in the Central-Western region of Brazil. However, livestock fed with these grasses can develop hepatogenous intoxication due to the presence of steroidal saponins that promote photosensitivity, inefficiency of production in the herds and death. Grass toxicity depends on factors related to animal susceptibility or resistance and intrinsic plant factors, providing significant differences in the clinical manifestation and mortality of livestock. Three experiments were conducted to investigate the adaptation management and inherited resistance to prevent Brachiaria spp. poisoning in sheep. In the first experiment, the adaptive management of lambs was performed, controlling the grazing time in Brachiaria spp. pastures. Groups of sheep grazing previously in Brachiaria decumbens paddocks for 2 h daily or on alternate days for 60 days presented low hepatotoxic changes and had reduced the number of intoxicated lambs when compared to the control group during the challenge. In experiment 2, ruminal transfaunation from adapted adult sheep to non-adapted lambs to grazing on the grass reduced the hepatotoxicity, which suggests that Brachiaria spp. poisoning resistance could be transferred by ruminal fluid to non-adapted animals. In the third experiment, lambs from flocks raised in Brachiaria pastures showed less susceptibility to poisoning than lambs from flocks raised in non-toxic pastures, suggesting inherited resistance to the toxicosis. These results suggest that the control of grazing time combined with transfaunation can be employed to decrease the frequency of poisoning in susceptible flocks. However, a definitive solution in the long-term would be the selection of resistant sheep herds.
650 $aOVEJA
650 $aOVINOS
653 $aPLATAFORMA SALUD ANIMAL
653 $aRESILIENCE OVINE
653 $aSIGNAL GRASS
653 $aTOXICITY RESISTANCE
700 1 $aGRACINDOA, C.V.
700 1 $aLANDIA, M.F.A.
700 1 $aABRAL FILHOA, S.L.S.
700 1 $aRESENDE FILHOA, N.J.
700 1 $aLIMAA, E.M.M.
700 1 $aRIET-CORREA, F.
773 $tSmall Ruminant Research$gv. 158, p. 42-47, 2018.
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
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Fecha actual : |
04/02/2020 |
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Actualizado : |
11/08/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
SILVEIRA, C.S.; ARMENDANO, J.I.; MOORE, D.P.; CANTÓN, G.J.; MACÍAS-RIOSECO, M.; RIET-CORREA, F.; GIANNITTI, F. |
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Afiliación : |
CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOAQUÍN IGNACIO ARMENDANO, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Balcarce, Buenos Aires, Argentina; DADÍN PRANDO MOORE, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina; GERMÁN JOSÉ CANTÓN, Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Balcarce, Buenos Aires, Argentina; MELISSA MACÍAS RIOSECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
A comparative study of commercial ELISAs for antibody detection in the diagnostic investigation of Neospora caninum-associated abortion in dairy cattle herds in Uruguay. [Comparación de ELISAs comerciales para la detección de anticuerpos en la investigación diagnóstica del aborto asociado a Neospora caninum en rodeos lecheros de Uruguay]. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Revista Argentina de Microbiología, April - June 2020, Volume 52, Issue 2, Pages 107-114. OPEN ACCESS. Doi: https://doi.org/10.1016/j.ram.2019.06.004 |
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ISSN : |
0325-7541 |
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DOI : |
10.1016/j.ram.2019.06.004 |
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Idioma : |
Inglés |
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Notas : |
Article history: Recibido 01 marzo 2019. / Aceptado 24 junio 2019. / Disponible online 28 de Noviembre de 2019.
Corresponding author: da Silva Silveira, C.; Instituto Nacional de Investigación Agropecuaria (INIA), Plataforma de Investigación en Salud Animal, Estación Experimental La Estanzuela, Colonia, Uruguay; email:carolsilveira7@hotmail.com
Los autores agradecen a los veterinarios que enviaron las muestras de suero e información adicional de los rodeos estudiados al laboratorio de la Plataforma de Investigación en Salud Animal de INIA La Estanzuela. |
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Contenido : |
ABSTRACT.
Bovine abortion causes considerable economic losses to the livestock industry worldwide and is of concern for public health and food safety, given that many abortigenic infectious agents of cattle are zoonotic. Despite its importance, the etiological diagnosis of abortion in cattle is challenging both for veterinary practitioners and laboratory technicians, partly due to the difficulty in recovering aborted fetuses under extensive field conditions for pathological and microbiological diagnostic investigation, and in the early identification of aborted dams. Neospora caninum is a cosmopolitan protozoon identified as one of the main abortigenic agents in cattle worldwide. In this study we propose a comparative seroepidemiological approach for the diagnosis of abortion by N. caninum in dairy cattle. Samples from 12 to 93 cows/heifers with and without recent history of abortion (cases and controls) in four commercial dairy farms were tested. The ratio of controls to the cases tested varied from 1:1 to 4.6:1. All samples (n = 230) were analyzed by three commercial ELISA kits for the detection of anti-N. caninum antibodies. In all four dairy farms, the proportion of seropositive cows and/or heifers per kit was significantly higher in the cases than in the controls (Odds Ratios = 5.13 to 36, p = 0.0002 to 0.0485). The agreement among the three kits varied from weak to strong (Cohe?s kappa coefficients = 0.58 to 0.83). We conclude that, despite the imperfect agreement between these kits, all of them allowed to arrive at similar conclusions regarding the statistical association between N. caninum seropositivity and abortion, thus representing a useful tool for the diagnostic approach at the population level under field conditions.
© 2019 Asociación Argentina de Microbiología
RESUMEN.
A pesar de su importancia, el diagnóstico etiológico del aborto en bovinos representa un desafío técnico para los veterinarios clínicos y laboratoristas, en parte debido a la dificultad de recuperar los fetos abortados en condiciones extensivas de campo. Dicha dificultad limita la posibilidad de detectar tempranamente los vientres abortados y de efectuar la investigación patológica y microbiológica de los abortos. Neospora caninum es un protozoario cosmopolita identificado como uno de los principales agentes abortigénicos en bovinos. En este estudio proponemos una aproximación seroepidemiológica para diagnosticar abortos por N. caninum en bovinos lecheros usando kits de ELISA comerciales. Se procesaron muestras de entre 12 y 93 vacas y/o vaquillonas con (casos) y sin (controles) historia reciente de aborto en 4 tambos comerciales de Uruguay. La proporción controles:casos analizados varió entre 1:1 y 4,6:1. Las muestras (n=230) fueron analizadas mediante 3 kits comerciales para la detección de anticuerpos IgG anti-N. caninum. En los 4 tambos la proporción de vacas y/o vaquillonas seropositivas por cualquier kit fue significativamente mayor en los casos respecto de los controles (odds ratio=5,13 a 36; p=0,0002 a 0,0485). La concordancia entre los kits varió de débil a fuerte (coeficiente Kappa de Cohen=0,58 a 0,83). Concluimos que, a pesar de la imperfecta concordancia entre estos kits, el empleo de todos ellos permitió arribar a conclusiones similares respecto de la asociación estadísticamente significativa entre seropositividad a N. caninum y aborto, lo que demuestra la utilidad de estos kits para la aproximación diagnóstica del aborto a nivel poblacional en condiciones de campo. MenosABSTRACT.
Bovine abortion causes considerable economic losses to the livestock industry worldwide and is of concern for public health and food safety, given that many abortigenic infectious agents of cattle are zoonotic. Despite its importance, the etiological diagnosis of abortion in cattle is challenging both for veterinary practitioners and laboratory technicians, partly due to the difficulty in recovering aborted fetuses under extensive field conditions for pathological and microbiological diagnostic investigation, and in the early identification of aborted dams. Neospora caninum is a cosmopolitan protozoon identified as one of the main abortigenic agents in cattle worldwide. In this study we propose a comparative seroepidemiological approach for the diagnosis of abortion by N. caninum in dairy cattle. Samples from 12 to 93 cows/heifers with and without recent history of abortion (cases and controls) in four commercial dairy farms were tested. The ratio of controls to the cases tested varied from 1:1 to 4.6:1. All samples (n = 230) were analyzed by three commercial ELISA kits for the detection of anti-N. caninum antibodies. In all four dairy farms, the proportion of seropositive cows and/or heifers per kit was significantly higher in the cases than in the controls (Odds Ratios = 5.13 to 36, p = 0.0002 to 0.0485). The agreement among the three kits varied from weak to strong (Cohe?s kappa coefficients = 0.58 to 0.83). We conclude that, despite the imperfect agreement betw... Presentar Todo |
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Palabras claves : |
Cohe?s Kappa coefficient; Neospora caninum; PLATAFORMA SALUD ANIMAL; SEROLOGÍA. |
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Thesagro : |
ABORT; BOVINOS; ELISA; EPIDEMIOLOGIA. |
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Asunto categoría : |
L72 Plagas de los animales |
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URL : |
https://www.elsevier.es/es-revista-revista-argentina-microbiologia-372-pdf-S0325754119300835
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Marc : |
LEADER 05374naa a2200325 a 4500
001 1060678
005 2020-08-11
008 2020 bl uuuu u00u1 u #d
022 $a0325-7541
024 7 $a10.1016/j.ram.2019.06.004$2DOI
100 1 $aSILVEIRA, C.S.
245 $aA comparative study of commercial ELISAs for antibody detection in the diagnostic investigation of Neospora caninum-associated abortion in dairy cattle herds in Uruguay. [Comparación de ELISAs comerciales para la detección de anticuerpos en la investigación diagnóstica del aborto asociado a Neospora caninum en rodeos lecheros de Uruguay].$h[electronic resource]
260 $c2020
500 $aArticle history: Recibido 01 marzo 2019. / Aceptado 24 junio 2019. / Disponible online 28 de Noviembre de 2019. Corresponding author: da Silva Silveira, C.; Instituto Nacional de Investigación Agropecuaria (INIA), Plataforma de Investigación en Salud Animal, Estación Experimental La Estanzuela, Colonia, Uruguay; email:carolsilveira7@hotmail.com Los autores agradecen a los veterinarios que enviaron las muestras de suero e información adicional de los rodeos estudiados al laboratorio de la Plataforma de Investigación en Salud Animal de INIA La Estanzuela.
520 $aABSTRACT. Bovine abortion causes considerable economic losses to the livestock industry worldwide and is of concern for public health and food safety, given that many abortigenic infectious agents of cattle are zoonotic. Despite its importance, the etiological diagnosis of abortion in cattle is challenging both for veterinary practitioners and laboratory technicians, partly due to the difficulty in recovering aborted fetuses under extensive field conditions for pathological and microbiological diagnostic investigation, and in the early identification of aborted dams. Neospora caninum is a cosmopolitan protozoon identified as one of the main abortigenic agents in cattle worldwide. In this study we propose a comparative seroepidemiological approach for the diagnosis of abortion by N. caninum in dairy cattle. Samples from 12 to 93 cows/heifers with and without recent history of abortion (cases and controls) in four commercial dairy farms were tested. The ratio of controls to the cases tested varied from 1:1 to 4.6:1. All samples (n = 230) were analyzed by three commercial ELISA kits for the detection of anti-N. caninum antibodies. In all four dairy farms, the proportion of seropositive cows and/or heifers per kit was significantly higher in the cases than in the controls (Odds Ratios = 5.13 to 36, p = 0.0002 to 0.0485). The agreement among the three kits varied from weak to strong (Cohe?s kappa coefficients = 0.58 to 0.83). We conclude that, despite the imperfect agreement between these kits, all of them allowed to arrive at similar conclusions regarding the statistical association between N. caninum seropositivity and abortion, thus representing a useful tool for the diagnostic approach at the population level under field conditions. © 2019 Asociación Argentina de Microbiología RESUMEN. A pesar de su importancia, el diagnóstico etiológico del aborto en bovinos representa un desafío técnico para los veterinarios clínicos y laboratoristas, en parte debido a la dificultad de recuperar los fetos abortados en condiciones extensivas de campo. Dicha dificultad limita la posibilidad de detectar tempranamente los vientres abortados y de efectuar la investigación patológica y microbiológica de los abortos. Neospora caninum es un protozoario cosmopolita identificado como uno de los principales agentes abortigénicos en bovinos. En este estudio proponemos una aproximación seroepidemiológica para diagnosticar abortos por N. caninum en bovinos lecheros usando kits de ELISA comerciales. Se procesaron muestras de entre 12 y 93 vacas y/o vaquillonas con (casos) y sin (controles) historia reciente de aborto en 4 tambos comerciales de Uruguay. La proporción controles:casos analizados varió entre 1:1 y 4,6:1. Las muestras (n=230) fueron analizadas mediante 3 kits comerciales para la detección de anticuerpos IgG anti-N. caninum. En los 4 tambos la proporción de vacas y/o vaquillonas seropositivas por cualquier kit fue significativamente mayor en los casos respecto de los controles (odds ratio=5,13 a 36; p=0,0002 a 0,0485). La concordancia entre los kits varió de débil a fuerte (coeficiente Kappa de Cohen=0,58 a 0,83). Concluimos que, a pesar de la imperfecta concordancia entre estos kits, el empleo de todos ellos permitió arribar a conclusiones similares respecto de la asociación estadísticamente significativa entre seropositividad a N. caninum y aborto, lo que demuestra la utilidad de estos kits para la aproximación diagnóstica del aborto a nivel poblacional en condiciones de campo.
650 $aABORT
650 $aBOVINOS
650 $aELISA
650 $aEPIDEMIOLOGIA
653 $aCohe?s Kappa coefficient
653 $aNeospora caninum
653 $aPLATAFORMA SALUD ANIMAL
653 $aSEROLOGÍA
700 1 $aARMENDANO, J.I.
700 1 $aMOORE, D.P.
700 1 $aCANTÓN, G.J.
700 1 $aMACÍAS-RIOSECO, M.
700 1 $aRIET-CORREA, F.
700 1 $aGIANNITTI, F.
773 $tRevista Argentina de Microbiología, April - June 2020, Volume 52, Issue 2, Pages 107-114. OPEN ACCESS. Doi: https://doi.org/10.1016/j.ram.2019.06.004
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